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A novel RP-HPLC method for the analysis of levetiracetam in formulations | Abstract

Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

ISSN: 0975-413X

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Abstract

A novel RP-HPLC method for the analysis of levetiracetam in formulations

Author(s): Narendra Devanaboyina, T.Satyanarayana and B.Ganga Rao

A simple, rapid RP-HPLC method was developed and validated for assay of Levetiracetam in formulations. Isocratic elution at a flow rate of 1.0ml/min was employed on a Chromosil C18 (250x4.6mm, 5μm in particle size) at room temperature. The mobile phase consisted of Methanol: Water:TEA 75:25:05 (V/V). The UV detection wavelength was 214 nm and 20μl sample was injected. The retention time for Levetiracetam was 2.59 min. The percentage RSD for precision and accuracy of the method was found to be less than 2%. The method was validated as per the ICH guidelines. The method was successfully applied for routine analysis of Levetiracetam in tablet dosage form and bulk drug.


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