Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

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A Pure Isolated from Fruit Seeds of Kolowe (Chydenanthus excelsus) The Identified as Single Peak By HPLC Analytic Column They Are Two Saponin Compounds of Isomered

Author(s): Laode Rijai, Hadi Kuncoro, Raymond Rumampuk, Unang Supratman, Husein Hernandi Bahti, Supriyatna and Wall Tylor

Analytical HPLC is a compounds separation tool that have better performances. The ability of the separation was not able to separate isomered saponins with physicochemical properties are very similar. It has been found two isomered saponins of an amorphous crystalline pure seed Kolowe (C. excelsus). The saponin separation using preparative HPLC column, and the results of analytical HPLC analytical column against the amorphous crystal showed a peak which indicates that it is a pure crystalline amorphous. However, the results of spectroscopic analysis with NMR-2D in a variety of techniques, the pure amorphous crystal is two saponins isomered. Two saponin are 3-O-[b-D-Xylopyranocyl(1®3)-b-D-glucopyranocyl(1®2)-b-D-glucuronopyranocyloxy]–28-O-(2- methylbuthyroyloxy)-oleana-12-en-15a, 16a, 22a-trihydroxy and 3-O-[b-D-Xylopyranocyl (1®3)-b-Dglucopyranocyl( 1®2)-b-D-glucuronopyranocyloxy]-28-O-(3-methylbuthyroyloxy)-oleana-12-en-15a, 16a, 22a- trihydroxy.


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