Triglycerides are a kind of lipids found in many food items such as vegetable oils which are rich sources of fats. These are metabolised by animal’s body easily but their increased concentration is dangerous for humans and can cause a variety of life threatening diseases like atherosclerosis, heart attack, stroke, pancreatitis nephritic syndrome, chronic hepatitis, hyper-lipidemia, liver disease, hypothyroidism and diabetes mellitus. There are many methods for the detection and determination of triglycerides. Traditional methods include chemical, enzymatic, fluorimetric and bioluminescent, chromatographic and HPLC and Flow injection analysis, these methods have certain limitations such as they are complex, require sample preparation due to which they are time consuming and also require trained personals. So, there is a great need of the new methods which can overcome these limitations. Biosensors provide the promising future for the easy and the early detection method. Role of biosensors in determining triglyceride concentration is the main highlight of this article. Lipase is the principle enzyme used in triglyceride determination; it produces glycerol, which is further oxidised enzymatically in several ways with glycerol dehydrogenase, glycerol-3-phosphate dehydrogenase, glycerol phosphate oxidase. Principle of working of biosensor, available supports for immobilisation and their advantages have been discussed. Even the sensitivity and performance of biosensors is being improved by using nano materials as transducer. Use of nanostructures like nanoparticle, nanotubes, nanorods, nanoprobes and their properties, merits over other substrates is also a part of the discussion. Recent research works done on triglyceride biosensor have been compiled in this review.