GET THE APP

A Validated RP-HPLC Method for Simultaneous Determination of Daidzein, Genistein, Formononetin and Biochanin A from In Vitro Cultured Cells of Trifolium pratense L. (Fabaceae) | Abstract

Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

ISSN: 0975-413X

ilbet

süperbetin giriş

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission Systemof respective journal.

agariookey oyna istanbul okey

Abstract

A Validated RP-HPLC Method for Simultaneous Determination of Daidzein, Genistein, Formononetin and Biochanin A from In Vitro Cultured Cells of Trifolium pratense L. (Fabaceae)

Author(s): Anil Kumar M., Vishnu Prasanth V. and Archana Giri

The objective of present study is to develop and validate a simple, precise, rapid, specific and accurate reverse phase HPLC method for the simultaneous determination of daidzein, genistein, formononetin and biochanin A obtained from in vitro cultured cells of Trifolium pratense L. Chromatographic separation was achieved on reverse phase octadecylsilane C18 column with isocratic elution. The mobile phase consisted of a mixture of ammonium acetate and methanol (40:60) pumped at a flow rate of 1.0 ml. min-1. The effluents were monitored at 254 nm with a UV detector at ambient temperature. Validation was carried out according to International Conference on Harmonisation (ICH) guidelines with respect to linearity, sensitivity, accuracy, precision, robustness and ruggedness. Good linearity was observed (r2 > 0.998) over the test concentration range of the four isoflavones. Precision, accuracy, limit of detection and limit of quantification obtained were within acceptable range in each case. The method was robust and specific for the four isoflavones. The results indicate that the developed method is simple, precise, rapid, specific and accurate, and would be suitable for the quantification of daidzein, genistein, formononetin and biochanin A obtained from in vitro cultured cells of Trifolium pratense L.


PDF

rokettube brazzers

https://pmasites.com/https://casinositeleriniz.com/https://fbhesq.com/

https://paperio-live.com

wowcappadocia.com
cappadocia-hotels.com
https://babesoflondon.com/
balloon-rides.net

wormax io