A reversed-phase high-performance liquid of chromatography (RP-HPLC) method for determination of two isomer sesquiterpene lactone deoxyelephantopin (1) and isodeoxyelephantopin (2) from Elephantopus scaber L. was developed and validated. Separation was achieved on a Phenomenex Luna C-18 (250 × 4.6 mm, 5 μm) column, with water: acetonitrile: 2-propanol (66:20:14, v/v/v) as mobile phase, flow rate of 1.0 mL-1 and UV detection was set up at 210 nm using DAD detector. Linearity for both compounds was found in the concentration range of 0.251-1.506 and 0.516-3.096 μg mL-1 (r2 ≥ 0.99), LOD is 0.094 and 0.151 μg mL-1, LOQ is 0.285 and 0.457 μg mL-1, and the range of recovery is 97.64-104.98% and 95.23-102.25%, respectively. The results of intra- and inter-day validation (n=3) showed the method was efficient and could be applied for the determination of sesquiterpene lactones in Elephantopus scaber L. extract.
Select your language of interest to view the total content in your interested language
Der Pharma Chemica received 11575 citations as per Google Scholar report