Abstract

An improved method for the detection of biogenic amines producing bacteria in fish

Author(s): Afilal Mohammed Elamin, Elasri Ouahid, Aseheaou Abd Salem and Daoudi Hanane

This work concerns the development of rapid and reliable method for the isolation and characterization of bacteria producing biogenic amines for two types of fishes, the sardine (Sardina pilchardus) and the wolf fish (Dicentrarchus labrax). We proposed new an experimental protocol with two steps: The first step searching the genes responsible for the production of biogenic amines the genetic methods, including the ERIC-PCR (Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction) and the Rep-PCR (Repetitive Extragenic Palindromic Polymerase Chain Reaction). The second step searching confirmation of the capacity of the genes identified to produce biogenic amines by chromatograph methods including the thin layer chromatography (TLC) for semiquantitative test and high performance liquid chromatography (HPLC) for quantitative test. The results found showed that the sequencing of these steps allows the isolation and the rapid and accurate characterization of detecting the biogenic amine producing bacteria in fish. The comparison of the two kinds of fish used confirms that sardine (red fleshed fish) disseminates the histamine producing bacteria more than the wolf (white fleshed fish), explaining the high involvement of sardine in histamine intoxication.

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