Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

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Analysis of differences in viral load results of DBS, DPS between the refrigerator and storage at a temperature of 37├?┬░C with plasma: A study in the blood of patients with HIV/AIDS in Jayapura, Indonesia

Author(s): Baiq Sunarniati, Daniel Lantang and Yohanis Ngili

The existence of a variety of advances in medicine and pharmaceuticals as well as primary prevention and secondary, such as several other countries, Indonesia has shifted policy focus on monitoring the viral load (VL) patients Anti Retroviral Therapy (ART) where previously the CD4 count is the main tool to monitor the effectiveness of treatment (CD4 count remains the criteria for determining the start of antiretroviral therapy in HIV-infected patients). Rationally this change is the accumulation of evidence of treatment failure where it is clinical or immunological criteria that cause problems delay the identification of viral failure that leads to the formation of resistant mutations. This type of research used in this research is the study sectional using data analysis analytically to describe the relationship the results of viral load / calculation of the amount of the HIV virus - RNA drops of dried blood/DBS (Dried Blood Spots) and DPS (Dried Plasma Spots) against Plasma. From this research we can conclude that there are differences in viral load results 37 ºC DBS, DBS Refrigerator, 37 ºC DPS, DPS Refrigerator and plasma viral load results. Based on the comparison of the average (mean) of the viral load results DBS 37 ºC, DBS Refrigerator, DPS 37 ºC, DPS Refrigerator and viral load results Plasma, then the result of the average value of viral load refrigerator is higher than the average value of viral load results 37 ºC, the average value of viral load results DPS more higher than the average value of viral load results DBS. While the average value of the results of plasma viral load higher than the average value of viral load results DPS. The results of this study open the cooperative research field of molecular biology and microbiology clinic to find the best way of sample storage.


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