Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry


Analysis of some anti-depressant drugs in aqueous samples using agarose film micro-electro driven membrane extraction

Author(s): Nor Suhaila Mohamad Hanapi, Mohd Marsin Sanagi,Wan Aini Wan Ibrahim, Nor’ashikin Saim, Abd Khamim Ismail, Wan Nazihah Wan Ibrahim, and Nur Diana Suhada Tahiruddin

Agarose film micro-electrodriven membrane extraction (AF-μ-EME) combined with liquid chromatography as a new microextractiontechnique was developed and applied for extraction of three selected anti-depressant drugs from tap water and human urine samples.Using 2-nitrophenyl octyl ether, NPOE as organic liquid membrane immobilized in the pores of a porous agarose film allowed the extraction of selective analytes from acidic sample solution into the acceptor phase. Different influencing parameters such selection of organic liquid membrane, sample pH, salt addition, extraction time and applied voltage were optimized.Under the optimal extractions conditions, the method demonstrated good linearity in the range of 0.5-500 μg L-1 withgood correlation of determinationr2 ≥ 0.9979,low limits of detection (0.02- 0.20 μg L-1), high enrichment factors (110-142), good relative recoveries in the range of (87-109.1%) and satisfactory precisions with RSDs of <8.9%(n = 3) were obtained. The validated method was successfully applied for the analysis of selected anti-depressantdrugs in tap water and human urine samples.