The essential oils of the flower were isolated by hydrodistillation from Perovskia abrotanoides karel, at different stages of plant growth. The oils were studied by GC/MS. The major components in the flower oils were: Camphor,1,8 – cineol, α-pinene, o-cimene, β-pinene, camphene, caryophyllene, α- humulene and borneol. The results show that, the oil yield and the major constituent's percentage of the flower were different at stages of plant growth. The essential oils of the flowers Perovskia abrotanoids Karel, were tested against two strains of Gram positive bacteria (Bacillus cereus, Staphylococcus aureus) and two strains of Gram negative bacteria (Escherichia coli, Klebsiella pneumonia). The average MICs and MBCs of essential oils of the flowers Provskia abrotanodies, were tested against the organisms by agar dilution.