Liver fibrosis is the excessive accumulation of extracellular matrix proteins including collagen that occurs in most types of chronic liver diseases. This study was carried out to evaluate the antifibrotic effect of ethanolic extract of whole plant of Cleome viscosa Linn. Liver fibrosis was induced by carbon tetra chloride (CCl4) administration in rats. The extent of liver fibrosis was assessed by measuring the level of liver hydroxyproline, thiobarbituric acid and serum enzymes levels. Following CCl4 administration, hydroxyproline, thiobarbituric acid levels were significantly increased and total platelet was decreased and serum enzymes levels were elevated. Treatment with two different doses of the ethanolic extract of Cleome viscosa Linn reduces hydroxy proline, thiobarbituric acid and also the serum enzyme levels. The liver weight that increased following CCl4 administration due to deposition of collagen was reduced by the ethanolic extract Cleome viscosa Linn. Thus the results showed that the ethanolic extract of whole plant of Cleome viscosa Linn found to possesses Antifibrotic effect and that evidenced by the biochemical parameters.