This study aims to investigate the antibiotic resistance of Escherichia coli O157 strains, producing Shiga toxins isolated from carcasse surface’s of one hundred and fifty (n=151) sheep. Indeed, there is a few data on the epidemiology of STEC O157 strains in Algeria. Thirteen strains of E.coli O157:H7 were isolated from 11 sheep carcasses (7.26%).The study of the sensitivity was tested with 31 antibiotics belonging to different classes, the disk diffusion method agar Mueller Hinton (CM337, Oxoid) using NCCLS standards (National Comittee for Clinical Laboratory Standards) recommended by WHO was used. The results showed that the three strains carrying the genes (eae, stx1, stx2) are resistant to tetracycline, a strain with (eae, stx2) genes to furans and a strain with (eae, stx2) genes, to furans and tetracycline. Eight strains were susceptible to all antibiotics tested. The presence of E. coli pathogens strains resistant to antibiotic results on a double public health problem. An extensive knowledge of STEC strains circulating and the introduction of monitoring plans to the various links in the food chain should be conducted at both veterinary and human level.