The ethyl acetate extract of Lantana camara L. was subjected in investigation of its antioxidant properties by DPPH method. The concentrated ethyl acetate extract of Lantana camara L. was column chromatographed by separation gradien polarity (SGP) method using silica gel as the stationary phase where is hexane, ethyl acetate and methanol selected as the mobile phases. Seven fractions were obtained, each fractions among them were subjected in investigation of its antioxidant properties and total phenolic content. This studies demonstrated thatIC50 value of Lantana camara L. extractwas found to be 36.18 mg/L with a total phenolic content was 2419.6 GAE. And IC50 value and total phenolic content of each fractions were found to be: Fraction A (132.62 mg/L and237.8 GAE), Fraction B (113.51 mg/L and589.4 GAE), Fraction C (85.23 mg/L and995.5 GAE), Fraction D (81.26 mg/L and1041 GAE), Fraction E (24.83 mg/L and3156 GAE), Fraction F (83.50 mg/L and1037.8 GAE), and Fraction G (806.71 mg/L). Based on the IC50, extract ethyl acetate and Fraction Eare highly active antioxidants, fraction C, D, and Fare active antioxidant, fraction A and B moderate antioxidant and fraction G is not active antioxidant. Antioxidant activity is influenced by total phenolic with correlation number was 98.14%.