During this work, the polyphenols in both Asteraceae Brocchia cinerea (Vis.) and Matricaria pubescens (Desf.) were targeted by two extraction methods, using different solvents. The extracts obtained by ethyl acetate were selected for column fractionation. Indeed, four fractions are obtained for M. pubescens and five for B. cinerea. The evaluation of the antioxidant capacity of these samples was carried out by two tests, commonly used in this field. The first one uses the free radical 1, 1-diphenyl-2 - picrylhydrazyl (DPPH) while the other (FRAP) uses 2,4,6-tris-2- pyridyl-s-triazine (TPTZ). These two tests revealed the radical scavenging power and reducing potential of both species B. cinerea and M. pubescens. Results have shown that the fraction obtained with the mixture EtOH/petroleum ether at (60/40) in the case of B. cinerea and that trained by 75% methanol in dichloromethane from M. pubescens, contained the majority of their active compositions. AcOEt extracts of both species and their fractions were tested against pathogenic bacterial strains, Gram-: Pseudomonas aeruginosa ATCC 27853, Enterobacter cloacae ATCC 13047 and Gram+: Staphylococcus aureus ATCC 25923, Enterococcus feacalis ATCC 29212. M. pubescens samples have exposed particular efficacy against E. cloacae and E. feacalis with minimal inhibitory concentration (MIC) estimated at 0.125 mg/ml.