To determine the phytochemical composition, anticancer activities and antioxidant of Nigella sativa methanol and ethyl acetate seed extracts. Phytochemical screening was characterized using standard qualitative and quantitative methods. The antioxidant activity was determined using the 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) and nitric oxide scavenging process whereas the in vitro cell viability and cytotoxicity was determined by using Breast Cancer Cell Line (MCF-7) cancer cells. Phytochemical screening of the extracts showing that they were rich in secondary class metabolite compounds in all three different solvents such as quionones, cardiac glycosides, terpinoides and phytosteroides and absence of saponins, phlobatannines and glycosides whereas tannins, flavonoids, phenols, coumarins were present only in Ethyl Acetate (EAC) and Methanol (MCE). Total phenolic and whole flavonoid composition were quantified and showed more in Methanol Extract (ME) than Ethyl Acetate Extract (EAE). In vitro antioxidant activity of DPPH radical scavenging showed 63.32 μg/ml in methanol extracts than in ethyl acetate extracts which found about 37.41 μg/ml. The nitric oxide scavenging activity of N. sativa showed 66.59 μg/ml in the methanolic extract and 36.66 μg/ml were found in EAE. Cytotoxic activity of 100 μg/ml concentrations of EAC and MCE exhibited 69.28% and 82.01% of cancer cell death respectively. The results show that N. sativa was a promising antioxidant and anticancer agent.
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