A simple, accurate and reproducible spectrophotometric method was established for the estimation of Rilpivirine by first derivative spectrophotometry. Methanol was used as the solvent. The proposed method obeys beer’s law in the concentration range of 6-16 μg/ml with maximum absorbance at 306 nm on the first derivative spectrum. The method was validated for accuracy, precision, ruggedness and robustness. The numerical values for all the validation parameters were within the acceptable limits. The results of rilpivirine were determined by linear regression equation with slope; 0.0047, intercept; 0.00066 and correlation coefficient of 0.9998. The recovery studies confirmed the accuracy of the proposed method and low values of standard deviation confirmed precision of the method. Spectral interference by excipients was eliminated by first order derivative spectrophotometric method. The proposed method was found to be suitable for regular analysis.
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