Biosynthesis and characterization of silver nanoparticles: Its use for bacterial detection | Abstract

Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

ISSN: 0975-413X
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Biosynthesis and characterization of silver nanoparticles: Its use for bacterial detection

Author(s): Priyanka Srivastava1, Shashank Pandey1*, Prashant Singh and Krishan Pal Singh

In this study; we have designed a Silver nanoparticles (AgNps) based piezoelectric Quartz crystal microbalance (QCM) AgNPs were synthesized and were coupled with anti sera of S. typhimurium. Coupled AgNps were characterized by TEM and FITR In TEM micrographs; we have observed spherical of AgNPs at the range ~50nm diameter Developed immunosensor for S typhimurium QCM has showed good reactivity and specificity towards its antigen of S. typhimurium; also showed decrease in frequency with respect to incubation time. The developed methodology is effective; selective; inexpensive and reproducible for the detection of S. typhimurium and a further step forwards towards development of point of care technology for the detection of bacteria Moreover; we have also demonstrated that the efficacy and sensitivity of sensors could be increased by immobilization of functional nanoparticles on the surface of quartz crystal


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