Biosynthesis of metal nanoparticle using fungi Claviceps paspali by varying composition of media, pH and temperature. The synthesized nanoparticle was characterized by UV-Visible spectroscopy, Scanning Electron Microscope (SEM) and Fourier Transform Infrared (FT-IR) Spectroscopy. Antimicrobial property of the nanoparticle was carried out against tested strains. Nanoparticle was also tested for their antidiabetic and anti-angiogenic properties. This work revealed that metal nanoparticle can be easily produced by fungi and may be a feasible, low-cost, environmentally friendly method for generating stable nanoparticles.