Bio-nanotechnology comprises the synthesis of nanoparticles through a reliable and eco-friendly process suitable for a necessary application such as a Green synthesis process, which is executed by the biological molecules in plant, extracts that. A versatile technique was implemented for the synthesis of Copper nanoparticle using an ethanol extract of Coriandrum sativum L. seeds. The reduction of cupper in aqueous solution of CuSO4•5H2O (2 mM) was confirmed by UV Vis spectrophotometer showing a typical resonance SPR (Surface Plasmon Resonance) at about 400-500 nm which is specific for the synthesis of nanoparticles (CuNPs). The formations of Copper nanoparticles from the Coriandrum sativum L. seed extract was identified first by observing the color changes, the color was changed from greenish brown to blue-brown color. The optimization of the various conditions, including, pH, time, the concentration of cupper sulfate pentagonal water CuSO4•5H2O, temperature and the mixing ratios of reactants to prepare the cupper nanoparticles (CuNPs) were studied also. Temperature 70ºC, and pH was 9.8, incubation time 30 min and the ratio of ethanolic extract 70% and cupper sulfate pentagonal water 25:75. X-ray Diffraction & Scanning Electron Microscope (SEM) analysis of cupper nanoparticles indicated that they exist in amorphous nature and the size of the particle lies in the range of 30-89 nm.
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