The antibacterial properties of plant extracts have been investigated in order to suggest them as potential tools to overcome the microbial drug resistance and the increasing incidence of foodborne diseases problems. The aim of this research is to study antibacterial activity of ethanol and hexane extracts from Tribulus Terrestris growing in Rabat of Morocco and Sa’adh of Yemen against ten pathogens bacteria. Soxhlet extraction technique was used to extract the organic extracts, using ethanol and hexane as solvent extract. Obtained extracts were analyzed by Gas chromatography (GC) and Gas chromatography-mass spectroscopy (GC-MS). GC and GC/MS results showed that oleic acid and stearic acid are major compounds of Tribulus Terrestris.