The oral administration of thiocolchicoside as skeletal muscle relaxant remains a less effective management of rheumatism and arthritis owing to poor oral bioavailability. Hence the present study was aimed to develop and evaluate nanoparticles containing thiocolchicoside as potential oral drug delivery system. The thiocolchicoside loaded chitosan nanoparticles were prepared by ionic gelation of chitosan with tripolyphosphate anions (TPP). The nanoparticles were characterized by Scanning Electron Microscopy (SEM), Zeta potential analyser, Fourier Transform Infra-red (FTIR) Spectroscopy and X- ray diffraction (XRD). All the prepared formulations resulted in nano range size particles (200 - 700 nm). The entrapment efficiency were found to be 74%- 81% respectively. The thiocolchicoside was found to be dispersed in the nanoparticles in microcrystalline polymorphic form. The invitro release profile of thiocolchicoside from the nanoparticles showed a sustained release of the drug over a period of 5 hrs.