The purpose of the research was to produce collagen hydrolysate from pangasius catfish skin and bone using bromelain enzyme. The resulted collagen peptides were expected to have antioxidative activity. Five different enzyme concentration ranged from 1-5% and different hydrolysis time (0, 5, 10, 20, 30, 40, 60, 90, 120 and 160 min) were used in the experiment. The parameters observed were yields of both skin and bone collagen, degree of hydrolysis and antioxidative activity (DPPH and reducing power). The result showed that collagen hydrolysate prepared by bromelain enzyme has antioxidative activity. The yield for both skin and bone collagen hydrolysate was 45.84% and 12.86% respectively. The highest degree of hydrolysis skin collagen hydrolysate was 2.58% at 160 minutes whereas the bone collagen hydrolysate had the highest degree of hydrolysis was 2.97% at 160 minutes. Based on the DPPH method skin and bone collagen hydrolysate had the antioxidative activity of 61.67% and 71.83%. In the other hand the reducing power method skin and bone collagen hydrolysate had the antioxidative activity of 0.35 and 0.38.
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