A highly sensitive and selective colorimetric method was developed for the determination of chondroitin sulfate in bulk drug and tablet dosage form. The proposed colorimetric method has been applied to quantify chondroitin sulfate by interacting with methylene blue. It is based on the principle of decrease in the absorbance of methylene blue at 664 nm on complexation with chondroitin sulfate. The Beer-Lambert’s law obeyed in the range of 0.5 - 4 mg/ml with correlation coefficient at 0.9985. The results presented are statistically validated in accordance with the guidelines provided by ICH. The recovery studies were carried out at three different levels. The precision was good with RSD lower than 2.0 %. The developed method was found to be considerably easy, simple, specific, precise, reproducible and cost effective.