Trichomonas gallinae is the flagellated protozoan parasite belongs to the category of zoomastigophorea, and order of trichomonadida. This parasite is located in the upper section of the gastrointestinal tract of numerous birds such as pigeons, chickens, eagles. Nitroimidazole compounds are carcinogenic, therefore is prohibited their use in veterinary medicine. . The aim of this study was to examine the effect of 6 herbals medicine extract on Trichomonas gallinae in vitro. The effect of different concentrations (1.25, 2.5 and 5 mg/ml) of extracts of Quercus Persica, Allium sativum, Artemisia annua, Myrtus commuis, Zataria multiflora, Rosmarinus officinalis was compared with metronidazole in 2, 4, 6, 18, 24, 48 and 72 hours after treating using trypan blue and eosin staining as vital stain. Sensitive of T. gallinae to the oak extract 2 mg/mL in 2 hours was more than the others and was close to the effect of metronidazole. In contrast to garlic extract did not show any sensitivity. The effect of the other extracts was between two mentioned extract. According to the results, the most effective extract on the trichomonas parasite was the oak fruit extract and then the rosemary extract. It is suggested, the study is also examined in vivo and if it was effective, used for the treatment of trichomoniasis in birds after the complementary studies.