Abstract

Determination of Fatty Acid Concentration and Profile in Breast Milk after Delivery

Author(s): Demet Özdemir, Necmettin Yilmaz, Sait Bulut

In this study, compositions of fatty acids were investigated in milk samples, collected within the first 20 days after delivery, containing colostrum, transition milk and mature milk periods of 24 newly delivered mothers. Mothers were divided into 2 groups and in the first group milk samples were collected from the same mother each day between 1-20 days and in the second group, which comprised of 23 mothers, the samples were collected in 5-10 days period for transition milk. Total lipid extracts were prepared from the collected milk samples. Methyl esters of fatty acids obtained from extracted total lipid samples were prepared and compositions of fatty acids were detected using gas chromatography. The most common fatty acids were palmitic acid, oleic acid, stearic acid, linoleic acid. While significant fatty acids as EPA and DHA were detected, some fatty acids were not detected at all. While fatty acid profile varied in milk samples of the groups, the difference was not statistically significant. The differences among breast milks can result from factor

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