Abstract

Determination of residues of deltamethrin in water and liver tissue of Zebrafish (Danio rerio) by HPLC

Author(s): Bangeppagari Manjunatha, G. Venkata Subba Reddy, Juan Ortiz Tirado, Patricia Falconí Salas, Darwin Rueda Ortiz

Deltamethrin is one of the most toxic pollutants for aquatic ecosystem. The present study investigated the extraction of deltamethrin in water and liver tissue of zebrafish (Danio rerio). Water was collected at 0, 24, 48, 72 and 96hrs after the addition of toxicant and the amount of deltamethrin present in the water and liver tissue. In the concentration level range of 5μg/L for the extraction of deltamethrin from water and liver tissue. Amount of deltamethrin in water and liver tissue analyzed by HPLC to know the amount of residue left out in the water and zebrafish liver after addition of 5μg/L of deltamethrin initially. The recoveries were the decrease of concentration deltamethrin in water and increase in liver with increase in time of exposure is an indication of the accumulation of the toxicant in the organism through uptake. This could be hazardous as it could make its way into the food chain and pose a potential ecological risk to the aquatic ecosystem and a consequent hazard to human health.

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