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Development and optimization of RP-HPLC method for the estimation of s (-) amlodipine in tablet dosage form | Abstract

Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

ISSN: 0975-413X

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Abstract

Development and optimization of RP-HPLC method for the estimation of s (-) amlodipine in tablet dosage form

Author(s): J. Bagyalakshmi, Sincy Mary Philip and T. K. Ravi

S(-) amlodipine is a potent calcium channel blocker used for the treatment of hypertension, congestive heart failure and angina pectoris. S(-) amlodipine avoids the adverse effect of amlodipine in racemic mixtures. The RP-HPLC method developed for the estimation of s (-) amlodipine in tablet dosage form was done by fixing the parameters as Phenomenex C8 ODS column (150 x 4.6 mm), 5 m particle size with mobile phase 20 mM sodium dihydrogen phosphate buffer: acetonitrile (65: 35% v/v) adjusted to pH 8 was used. Mobile phase flow rate was maintained at 1.2ml/min and detected at 239nm. The retention time was 4.20± 0.02 minutes


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