GET THE APP

Development and validation of a RP-HPLC-PDA method for simultaneous estimation of Drotaverine and Etoricoxib in tablet and its application for dissolution studies | Abstract

Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

ISSN: 0975-413X
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission Systemof respective journal.

Abstract

Development and validation of a RP-HPLC-PDA method for simultaneous estimation of Drotaverine and Etoricoxib in tablet and its application for dissolution studies

Author(s): P. K. Syal, M. Sahoo, K. D. Ingale, S. S. Ingale, V. P. Choudhari and B. S. Kuchekar

A simple, precise, rapid and accurate RP-HPLC-PDA method has been developed for the simultaneous estimation of Drotaverine HCl (DRT) and Etoricoxib (ETR) in tablet formulations. The chromatographic separation was achieved on Waters Kromosil C18 column (250 mm x 4.6 mm, 5.0 μ particle size) using methanol: THF: acetatebuffer (51:09:40 v/v) pH adjusted to 6.0 with acetic acid, flow rate was 0.9ml/min and column was maintained at 55 0C. Quantification and linearity was achieved at 244 nm over the concentration range of 1.6 – 80μg/ml for Drotaverine HCl and 1.8 – 90μg/ml for Etoricoxib. The method was validated for specificity, linearity, accuracy, precision, LOD, LOQ and robustness. The proposed method was optimized and validated as per the ICH guidelines. Method was used to test dissolution sample successfully. Thiocolchicoside and Hydrochlorthiazide were used as internal standard.


PDF

Select your language of interest to view the total content in your interested language

30+ Million Readerbase
SCImago Journal & Country Rank
Google Scholar citation report
Citations : 25868

Der Pharma Chemica received 25868 citations as per Google Scholar report

Der Pharma Chemica peer review process verified at publons
Der Pharma Chemica- Journals on pharmaceutical chemistry