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Development and validation of a RP-HPLC-PDA method for simultaneous estimation of Hydrochlorothiazide and Irbesartan | Abstract

Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

ISSN: 0975-413X
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Abstract

Development and validation of a RP-HPLC-PDA method for simultaneous estimation of Hydrochlorothiazide and Irbesartan

Author(s): Aniruddha R. Chabukswar, Swati C. Jagdale, Bhanudas S. Kuchekar, Pradeep D Lokhande, Santosh N. Shinde, Kunal D. Ingale, Anuja K. Kolsure

A simple, precise, rapid and accurate RP-HPLC-PDA method has been developed for the simultaneous estimation of Hydrochlorothiazide and Irbesartan in tablet formulations. The chromatographic separation was achieved on Waters Symmetry C18 column (250 mm x 4.6 mm, 5.0 μ particle size) using methanol: THF: acetate buffer (60:10:30v/v) pH adjusted to 5.5 with acetic acid, flow rate was 0.75 ml/min and column was maintained at 500C. Quantification and linearity was achieved at 271 nm over the concentration range of 0.3 – 50 μg /ml for Hydrochlorothiazide and 3.6 - 600 μg/ml for Irbesartan. The method was validated for specificity, linearity, accuracy, precision, LOD, LOQ and robustness. The proposed method was optimized and validated as per the ICH guidelines.


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