GET THE APP

Development and validation of a stability indicating RP-HPLC method for the determination of trimetazidine dihydrochloride | Abstract

Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

ISSN: 0975-413X
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission Systemof respective journal.

Abstract

Development and validation of a stability indicating RP-HPLC method for the determination of trimetazidine dihydrochloride

Author(s): Sreelatha Nagula, Sridhar Thota* and Venisetty Raj Kumar

A stability-indicating RP-HPLC method was developed and validated for the determination trimetazidine in active pharmaceutical ingredient and tablet dosage form. The chromatographic conditions comprised of a reversed phase Enable C18G column (250mm×4.6μm, 5μ), with a mobile phase consisting of methanol-0.05% formic acid (90:10 v/v) with flow rate of 1.0 ml/min. Detection was carried out at 232 nm. The retention time of trimetazidine was found to be 3.8 min. The linear regression analysis data for the calibration plots showed good linear relationship within the concentration range 10–80 μg/ml. The value of correlation coefficient was found to be 0.999. The recovery of trimetazidine hydrochloride was about 95–105%. Trimetazidine dihydrochloride was subjected to stress conditions including acidic, alkaline, oxidation, photolysis and thermal degradation. Trimetazidine is more sensitive to wards acidic degradation. The method was validated as per ICH guidelines.


PDF

Select your language of interest to view the total content in your interested language

30+ Million Readerbase
SCImago Journal & Country Rank
Google Scholar citation report
Citations : 25868

Der Pharma Chemica received 25868 citations as per Google Scholar report

Der Pharma Chemica peer review process verified at publons
Der Pharma Chemica- Journals on pharmaceutical chemistry