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Development and validation of novel UV spectrophotometric determination of levofloxacin hemi hydrate in bulk and pharmaceutical dosage forms | Abstract

Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

ISSN: 0975-413X

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Abstract

Development and validation of novel UV spectrophotometric determination of levofloxacin hemi hydrate in bulk and pharmaceutical dosage forms

Author(s): Manimala M., Ravindra Reddy K. and Hepcykala Rani D

The objective of this research was to develop and validate a simple, rapid, accurate and economical UV Spectrophotometric method for determination of levofloxacin hemi hydrate in bulk and marketed formulation. In Water, the λmax of the drug was found to be 286.4 nm. In the proposed method, levofloxacin hemi hydrate follows linearity in the concentration range 4 –10 μg/ml with a correlation coefficient (R2) greater than 0.997 .The method was validated by following the analytical performance parameters suggested by the International Conference on Harmonization (ICH). All validation parameters were within the acceptable range. Under experimental conditions described, calibration curve, assay of tablets and recovery studies were performed. Parameters of validation prove the precision of the method and its applicability for the determination of Levofloxacin Hemi hydrate in pharmaceutical tablet formulations. The method is fast and is suitable for high throughput analysis of the drug.


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