Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry


Development and validation of rapid HPLC method for determination of Pregabalin in bulk drug and capsule dosage forms

Author(s): Ashu M*, Parmar S, Nagarajan K and Vijendra Singh

A simple,selective, rapid, and economical reversed phase high performance liquid chromatography(RP-HPLC)method for the determination of pregabaline in the capsule dosage form has been developed and validated.The separation and quantification were achieved on waters spherisorb 5μ ODS 24.6mm x 250mm column using a mobile phase of acetonitrile:buffer (30:70 V/V) at a flow rate of 1ml/min with detection of analyte at 210 nm. The separation was achieved within 3.1±0.3 min for pregabalin sample. Peak width was found to be 5.26s and SD was found to be 1.3152 for the sample peak . The method showed good linearity in the range of 200-800 μg/ml. The intra and inter day R.S.D ranged from 0.79-1.85% The recovery (mean ±S.D.) of low, middle and high concentrations were 100.02 ± 0.80, 100.05 ± 0.42, 100.03 ± 0.35 respectively.