Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

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Development and Validation of RP-HPLC and HPTLC methods for Estimation of Ritonavir in bulk and in pharmaceutical formulation

Author(s): T Sudha, R Vanitha and V Ganesan

This research paper describes validated reverse phase high performance chromatography (HPLC) and high performance thin layer liquid chromatography (HPTLC) methods for the estimation of Ritonavir in bulk and in pharmaceutical formulation. The RP-HPLC separation was achieved on Eclipse XBD (C18) RP column (150mmX 4.6mm id, 15μparticle size) using acetonitrile: water (60:40%v/v) as the mobile phase at a flow rate of 1.4ml/min at an ambient temperature. Quantification was achieved with ultraviolet (SPD-10AV) detection at 209 nm over the concentration range 5-30μg/ml with recovery range 99.73 to 101.39% for RTV by HPLC method. The HPTLC separation was achieved on the aluminum backed layer of silica gel 60F254 using (Toluene: ethyl acetate: methanol: glacial acetic acid) (7.0:2.0:0.5:0.5%v/v/v/v) as mobile phase. Quantification was achieved with HPTLC detection at 263nm over the concentration range of 200 to 1000 ng/ spot with recovery in the range of 98.00-101.11% for RIV. Both the methods are simple, precise, and sensitive and extended for routine analysis in bulk as well as pharmaceutical formulation


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