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Development and validation of RP-HPLC method for the analysis of carbofuran and in its formulations | Abstract

Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

ISSN: 0975-413X

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Abstract

Development and validation of RP-HPLC method for the analysis of carbofuran and in its formulations

Author(s): K. Vinod Kumar and N. V. S. Naidu

A simple, selective, precise and accurate High Performance liquid Chromatographic method for the analysis of Carbofuran in its formulations was developed and validated in the present study. The mobile phase consist a mixture of Acetonitrile and Potassium Dihydrogen Orthophosphate in the proportion 60:40 and adjust the pH to 5.8. This was found to give sharp peak of Carbofuran at a retention time of 4.052 min. HPLC analysis of Carbofuran was carried out at a wave length of 282 nm with a flow rate of 1.0μL/ min. the linear regression analysis data for the calibration curve showed a good linear relationship with a regression coefficient 0.999 in the concentration range of 7.5μg to 75μg. The linear regression equation was y = 131065x + 16835. The developed method was employed with a high degree of precision and accuracy for the analysis of Carbofuran. The method was validated for accuracy, precision, robustness, Ruggedness, Specificity. The Precision, accuracy, sensitivity, short retention time and composition of the mobile phase indicated that this method is better for the quantification of Carbofuran.


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