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Development and Validation of UV Spectrophotometric Method for Estimation of Lamivudine in Bulk and Tablet Dosage Form | Abstract

Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

ISSN: 0975-413X
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Abstract

Development and Validation of UV Spectrophotometric Method for Estimation of Lamivudine in Bulk and Tablet Dosage Form

Author(s): M Akif Haque, Kuchukuntla Mounika, Vasudha Bakshi

UV Spectrophotometric method has been developed for estimation of lamivudine in bulk and tablet dosage form by using water as solvent. Method developed is Area Under Curve (AUC) method. For analysis of lamivudine by AUC method the wavelength range selected was 265 nm to 275 nm because the linearity was obtained within this area with good reproducibility of results. Lamivudine shows absorbance maxima (λmax) at 271 nm scanned in UV range of 400 nm to 200 nm. Lamivudine obeys Beer’s law in the concentration range of 10-60 μg/ml.


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