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Development and validation of UV spectrophotometric method for the estimation of asenapine maleate in bulk and pharmaceutical formulation | Abstract

Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

ISSN: 0975-413X

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Abstract

Development and validation of UV spectrophotometric method for the estimation of asenapine maleate in bulk and pharmaceutical formulation

Author(s): Halima O. A, Aneesh T. P, Reshma Ghosh, Nathasha. R. Thomas

A simple, accurate, precise and sensitive UV spectrophotometric method was developed for the determination of Asenapine maleate in bulk and pharmaceutical dosage form. The solvent used is methanol and the wavelength corresponding to maximum absorbance of the drug was found at 270nm. Beers law was observed in the concentration range of 10- 60μg/ml with correlation coefficent 0.9997. The linear regression equation obtained by least square regression method were y=0.0132X-0.0016, where y is the absorbance and x is the concentration of the pure drug solution. The method was validated for several parameters like accuracy, precision as per ICH guidelines. The values of relative standard deviation and % recovery were found to be satisfactory, indicating that the proposed method is precise and accurate and hence can be used for the routine analysis of Asenapine maleate in bulk and pharmaceutical formulation.


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