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Development and validation of UV-Spectrophotometric method for quantitative determination of inulin by specific absorbance | Abstract

Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

ISSN: 0975-413X

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Abstract

Development and validation of UV-Spectrophotometric method for quantitative determination of inulin by specific absorbance

Author(s): Оlga А.Yevtifieieva, Kseniya I. Proskurina, Nataliya N. Smelova and Irina Yu. Petukhova

The sensitive, accurate and precise spectrophotometry method by specific absorption of quantitative determination of the inulin substance after acid hydrolysis to the5-hydroxymethylfurfural(5-HMF) has been developed. The specific absorption of 5-HMF, which is equal to 258±2.86, has been determined experimentally. The validation characteristics of the proposed method (linearity, accuracy, precision, robustness) have been studied. The estimation of validation characteristics of the quantitative determination methods of inulin substance by the specific absorbance allows us to conclude that using the method is correct for routine analysis.


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