Threesimple and sensitive spectrophotometric methods (A, B and C) have been developed forthe quantitative determination of Mesalamine in bulk drug and pharmaceutical preparations.Method A, B are based on the condensation of Mesalamine with p-dimethyl aminobenzaldehyde and pdimethyl amino cinnamaldehyde to form schiff’s bases, to yield a yellow, red colored chromogen and exhibits absorption maxima at 440, 523.5 nm. Method C isbased on the reaction of Mesalamine with Folin-Ciocalteu (phenol’s) reagent under alkaline conditions forming a blue colored chromogen and exhibits absorption maxima at 616 nm. Beer’s law was obeyed inthe concentration range of 50-250, 20-100 and 10-50μg/ml for method A,B and C respectively. Thesemethods were extended to pharmaceutical formulations and there was no interference from any common excepients. The results of analysis have been validated statistically and by recoverymethods.