Development of RP-HPLC method for simultaneous estimation of lactic acid and glycolic acid | Abstract

Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

ISSN: 0975-413X
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission Systemof respective journal.


Development of RP-HPLC method for simultaneous estimation of lactic acid and glycolic acid

Author(s): Ginjupalli Kishore, Armugam Karthik, Shavi Venkat Gopal, Averineni Ranjith Kumar, Mahalingam Bhat and N. Udupa

Quantification of the acidic monomers of biodegradable polymers plays an important role in understanding the degradation process. Aim of the present study was to develop a specific and accurate reversed phase high performance liquid chromatographic method (RP-HPLC) for the quantification of lactic acid (LA) and glycolic acid (GA) using conventional C18 column with ultra-violet detection. Mobile phase comprised of phosphate buffer (10 mM, pH 3.0) and acetonitrile at a ratio of 95:5 %v/v which resulted in the separation of LA and GA within 5 min run time. The stationary phase was conventional C18 column (250 x 4.6 mm, 5 μm). The detection was carried out at UV wavelength of 210 nm and the retention time was found to be 3.11 and 3.69 min for GA and LA respectively. The calibration curve was linear for the selected range with a coefficient of determination 0.9992 ± 0.0021 and 0.9975 ± 0.0015 for GA and LA respectively. The proposed method is sensitive, simple and cost effective compared to the previously reported methods utilizing the special columns. The developed method was validated and the recoveries of GA and LA were 98.51 ± 3.46% and 97.20 ± 2.84% respectively. The developed method can be very useful for the estimation of LA and GA during the degradation of polymers based on these acids.


Select your language of interest to view the total content in your interested language

30+ Million Readerbase
SCImago Journal & Country Rank
Google Scholar citation report
Citations : 25868

Der Pharma Chemica received 25868 citations as per Google Scholar report

Der Pharma Chemica peer review process verified at publons
Der Pharma Chemica- Journals on pharmaceutical chemistry