The antibacterial, antifungal and anti-oxidant properties of the Garden thyme herb are well known. The aim of this study was to examine the effect of thyme aqueous extract on the serum levels of creatine kinase (CK) and lactate dehydrogenase (LDH) in Inactive women following a single session of exhaustive exercise. subjects used in this semi experimental study included 32 female students, mean age of 21.6± 1.41 years, height of 172.66± 4.63 cm and weight of 80.25±11.77 kg. They were randomly divided into 4 groups including: the control, experimental group 1 receiving 200 mg/kg thyme aqueous extract, the experimental group 2 receiving 400 mg ibuprofen and the experimental group 3 receiving both the extracts and ibuprofen. Ibuprofen tablet and the extract were orally administered 8 hours after exhaustive exercise. Physical activity program for inducing muscle soreness was 70 contractions of bicep muscles; each repetition was 40-60% of a maximum contraction. The CK and LDH parameters were measured both before as well as 24, 48 and 72 hours after the exhaustive exercise. According to the results, there were no significant differences in CK and LDH serum levels among different groups at 24, 48 and 72 hours after exhaustive exercise. The results of this study suggest that the scope and extent of muscular damage during sport activities depends on a number of factors, including the severity, duration and type of exercise as well as the type, degree of purity, the amount and the time of supplement consumed.