The need for sustainable energy sources has led to the increased investigations over Li-ion batteries (LIBs). In order to achieve high power and long life LIBs, various electrode materials are studied in the recent past. Among them, spinel lithium titanate (Li4Ti5O12) is an interesting anode material and is a potential candidate owing to its unique zero strain insertion nature. In this work, efforts have been made to synthesize Li4Ti5O12 (LTO) nanoparticles solvothermally, using TiO2 and LiOH.H2O as the starting materials in the presence of ethanol /water mixture at two different reaction conditions, based on time viz. 12 h and 24 h. These two samples are labeled as LT-12 and LT-24, respectively. The crystallinity of the as-prepared LTO was confirmed from X-ray Diffraction (XRD) patterns. The XRD pattern of LT-24 has more intense peaks in comparison to LT-12, indicating better crystallinity of the Li4Ti5O12 nanoparticles obtained. The surface morphology of both LT-12 and LT-24 was identified from Field Emission Scanning Electron Microscopy (FESEM). The High Resolution Transmission Electron Microscopy (HRTEM) imaging illustrates the cubic shape of LTO nanoparticles. The vibrational frequencies of elements present in LTO are obtained from Fourier Transform Infrared (FTIR) spectra. The Electrochemical Impedance Spectroscopy (EIS) was used to study the electrochemical performance of Li4Ti5O12 spinels. The shape of impedance curve depicts both the charge transfer impedance and diffusion of Li-ions and validates the better use of LT-24 as anode material for LIBs.