Flavonoids are polyphenolic secondary metabolites produced by the plants. They are extensively studied within drug discovery programs due to their beneficial and pharmacological activities such as antioxidant, antimicrobial, antitumor, antidiabetic and cardio-protective. The ability of flavonoids to coordinate with metal atoms has presented new leads for drug innovation programs, with enhanced pharmacological activities and clinical outputs than the parent flavonoids. Recently, we have reported the synthetic method, spectral analysis and antidiabetic properties of a new Zinc –Avicularin complex. The present effort was focused on the DNA binding of Avicularin and its ternary transition metal complex with Zinc. The nature of the binding interactions between complex and calf thymus DNA (CT-DNA) was studied by electronic absorption spectra, fluorescence spectra, circular dichroic and viscosity measurements. The data obtained evidence that the Avicularin and its Zn (II) complex could interact with CT-DNA via partial/moderate intercalative mode.