This study was carried out to evaluate some enzymes activity in hydatidosis infection which were considered as a biomarkers to monitor their levels and prognostic values. They were performed on 50 blood samples of hydatidosis surgically proved and 20 samples as control group which were collected between October, 2015 and April, 2016 from different hospitals in Al-Furat Al-Awsat district. The mean levels of alkaline phosphatase (ALP) and lactate dehydrogenase (LD) exhibited a significant elevation (P < 0.05) in hydatidosis patients compared to the healthy control group. Also the same findings were seen in pre-operative patients compared to post-operative patients. The levels of both enzymes increased in liver and multi-organs hydatidosis than any other site. The patients with large size cyst showed a significant increase (P < 0.05) in ALP and LD levels compared to small size cyst. There was a significant decrease(P < 0.05) in the levels of adenosine deaminase (ADA) and glutathione reductase (GHR) enzymes in hydatidosis patients compared to healthy control. The ADA levels in large cyst were significantly increased (P < 0.05) compared to patients with small cyst. The (GHR) enzyme level was decreased in patients with small size cyst compared to the large ones. Also the multi-organs and liver hydatidosis showed significant decrease (P < 0.05) in the levels of GHR compared to lung or kidney.