Abstract

Evaluation of vitamin E on microscopic parameters of chilled and frozen stored ram semen

Author(s): Mahnaz Ahmadi Hamedani, Abdol-Mansour Tahmasbi, Abbas-Ali Naserian and Yousef Jafari Ahangari

The production of reactive oxygen species during the process of cryopreservation, decrease the motility and cellular viability by initiation of lipid per oxidation of bio-membranes. For reduce these damages, antioxidants are used as cryoprotectants against the lipid per oxidation. The aim of this study was to assess the influence of vitamin E adding to the dilution media on standard qualitative parameters (motility, progressive motility, viability, hypo-osmotic swelling test, Acrosomal damages and normal spermatozoa) of pre and post frozen-thawed ram semen. Semen samples were collected by Electro ejaculator from 6 Zel rams, and diluted with a Tris-base extender containing vitamin E (1, 2 and 3 m/M) and without antioxidants (control). Diluted semen was cooled to 5oC and frozen in 0.25 ml straw, prior to being stored in liquid nitrogen. The results show that vitamin E has positive protection effects on the semen characteristics in chilled and frozen thawed. Motility, progressive motility, viability, hypo-osmotic swelling test and total normality of spermatozoa were higher in all groups of vitamin E supplement compare with control group, but highest percentages of treats were observed in 2m/M of vitamin E. Also the effect of vitamin E on Acrosome damages were significant in post frozen conditions and the highest percentages were obtained in control group. Therefore we recommend using 2m/M of vitamin E in Tris extender for short and long storages of Zel ram spermatozoa.

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