This work aims at antibacterial testing of calcium chloride cross-linked starch and chitosan (50:50) biodegradable blended films. The blended films are incorporated with 1%, 2%, 3% and 4% of two different natural fibers, Jute and coir. The prepared blended films are subjected to test the antibacterial property against Escherichia coli.
No bacterial growth on the surface of jute and coir fiber added blended films are found. Besides, one attention-grabbing thin line (hair like) of zone of inhibition is observed around 1% jute fiber added blended film. Furthermore, to boost the antibacterial effect of jute and coir blended films, 2 gm of dry Azadirachta indica (neem) leaf powder is added to each 50 gm of the starch-chitosan blend containing 1% jute fiber and 1% coir fiber. The antibacterial property of blended films is observed after 24 hours. Zone of inhibition for neem leaf powder added 1% jute fiber blended film get extended to 18 to 20 mm while microbial growth is not observed on the surface of Azadirachta indica leaf powder added 1% coir fiber blended film. All the results are observed under microscope Nikon Eclipse Ti 60 X.