Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

Abstract

Extraction, Phytochemical, Antibacterial Screening and Spectroscopic Analysis of the Crude Samples of Stem Bark Extract of Lonchocarpus cyanescens

Author(s): Nwokonkwo DC, Okpani AN, Elom N

Powdered stem bark of Lonchocarpus cyanescens was batch extracted with chloroform, methanol and water to yield varying proportions of the extracts: Stem Bark Aqueous Extract (SAE), Stem Methanol Extract (SME) and Stem Chloroform Extract (SCE) respectively. The thin-layer chromatography revealed 5-6 constituents for each extract. The extracts were each washed in a column packed with alumina using n-butanol-glacial acetic acid-water (5:1:4) upper layer and screened for the presence of phytochemicals. The extracts indicated the presence of alkaloids, saponins, tannins, cardiac glycosides, flavonoids and phenols. The ultraviolet-visible (UV-VIS) spectroscopy of SCE showed peaks between 650-776.0 nm, SME between 497.0-795.5 nm and SAE showed bands between 208.5-760.1 nm. The Infrared Spectroscopy (IR) of SCE showed significant broad stretches at 3444.03 cm-1 for the presence of hydrogen bonds (OH), 2932.50 cm-1 C=C of aromatic compounds, 1645 cm-1 for aromaticity and overtoned at 1186 cm-1. The IR of SME showed identical peaks at 3442.09 cm-1. 2931.90 cm-1, 1644.37 cm-1 and 1063.78 cm-1. The IR peaks or bands of SAE appeared at 3431.48 cm-1, 2944.44 cm-1, 1634.73 cm-1 and 1096.57 cm-1. The Gas Chromatography-Mass Spectrometry (GC-MS) of SCE showed a molecular peak (m/z) at 282, SME at 206 and SAE at 256. The antimicrobial activity of the extracts showed sensitivity at a concentration as low as 1.0 × 10-4 mg/mL against four human pathogens; Pseudomonas aeruginosa, Escherichia coli, Salmonella typhi and Staphylococcus aureus. The extracts did not inhibit the growth of Klebsiella pneumonia and Streptococcus pneumonia.


PDF

FIND ARTICLES
SCImago Journal & Country Rank
 
Erkek öyle sert ve acımaz sikiyor ki bayan çaresiz bir şekilde tırtıl misali sikiş yataktan aşağı doğru kayıyor fakat hem adam hemde kadın aşırı mobil porno derecede zevk alıyorlar Uzun zamandır karısı ile ilgili planları olan türk porno adam onu başkaları sikerken hayal ediyor bu durumu karısından sikiş izle gizliyordu fakat bir gün adamın moralinin bozuk olduğunu anlayan karısı sikiş ona ne olduğunu sordu ve adamda ona her şeyi anlatmıştı karısı bu porno resim durumdan Büyük zenci yarağı sevenler için özel olarak eklenmiş videomuz türk porno kalın ve damarlı zenci siki arayıp bulamayanlar için özel olarak sikiş izle yayınlanmıştır filmde ise sarışın zengin sürtük büyük mobil sikiş kalın damarlı alet sevdiğini bir çok kez dile getirmiş