Carica papaya is a domestically produced plant and it is an accepted medicinal plant which is used traditionalistic and domesticated in India and tropical area of this world. The present study describes phytochemical constituent of Carica papaya leaves and its antibacterial screening. Fresh leaves sample were collected during the month of February, 2018 from Gautam Buddha University and Dadri (UP), India. The purpose of the study was the effect of different solvent and different method on the yield of the bioactive compounds of the papaya leaves growing in the semiarid region of Uttar Pradesh. Grinded leaves of papaya were extracted using different sovent i.e., n-hexane, PET, water, alcohol (ethanol, methanol), hydroalcohol (water+methanol) and the extraction was done by different technique soxhlet, as well as microwave assisted extraction technique. Many of these phytochemicals have amazing effects on long-term health when consumed by the human and can be efficaciously used to treat human diseases. The TLC analysis and phytochemical screening was carried out using standard methods and protocols. This study demonstrates that the microwave assisted extraction technique is better than soxhlet technique it also shows that hydro-alcoholic leaf extract of papaya gives better yield by microwave assisted extraction method and extracted leaves extract also shows the antibacterial activity against E. coli and Bacillus subtilis.