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Fast and Simple Method for Assay Determination of Metformin and Glyburide from Combination Tablet Dosage form by UV Spectrophotometer | Abstract

Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

ISSN: 0975-413X

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Abstract

Fast and Simple Method for Assay Determination of Metformin and Glyburide from Combination Tablet Dosage form by UV Spectrophotometer

Author(s): Sushama Ambadekar, Sameer S Keni, Deepak B Nikam

Fast, simple, economic and accurate methods have been developed for simultaneous determination of assay of metformin and glyburide from combination tablet dosage form. UV-Visible spectrophotometric method is developed with measurement of absorption at maximum wavelength of 233 nm and 301 nm for metformin and glyburide respectively. The developed method was validated and proved to be precise and robust. UV responses were found to be linear in the concentration range of 8 to 12 ppm for metformin and 80-120 ppm for glyburide. The correlation coefficient was found to be 0.9994 for metformin and 0.9998 for glyburide. The mean percentage recovery was found to be 99.8% for metformin and 99.9% for glyburide. This method will be useful for the determination of assay in combination tablet dosage form.


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