To achieve successful colonic delivery, a drug needs to be protected from absorption or the environment of the upper gastro intestinal tract and then be abruptly released into proximal colon, which is considered the optimum site for colon-targeted delivery of drugs. Most of the conventional drug delivery system for treating various disorders and diseases such as inflammatory bowel disease, colon cancer and intestinal amoebiosis have failed as drugs in its intact form as they do not reach the site of action in appropriate concentration. For an effective and safe therapy for such diseases, development of a specific drug delivery system is a challenging task. Hence we aim to develop a drug delivery system which would deliver the drug in its intact form as close as possible to target site, leading to reduction of dose related side effects. The objective of present study was to develop multiparticulate colon drug delivery system of Celecoxib. The colon drug delivery system of Celecoxib microspheres was prepared by emulsion polymerisation method by using ethyl cellulose and Eudragit S 100 polymers in varying concentration. Formulations were evaluated for percent yield, entrapment efficiency, scanning electron microscope, FTIR spectroscopy and in vitro release studies. The optimized formulations of Celecoxib microspheres shows to prolonged activity with increased stability without loosing its therapeutic activity in colon drug delivery system.