Free radical copolymerization of 4-Cl (phenyl) maleimide (N-PCPMI) with ethyl acrylate (EA) was performed at 70°C using THF as a solvent and BPO as a free radical initiator. Copolymer is characterized by nitrogen percentage intrinsic viscosity, solubility test, FT-IR, 1H NMR spectral analysis. Variations based on solvent, free radical initiator, time was also studied. The intrinsic viscosity of C-PCPMI II in DMF solution was 0.170 dL/g. Thermogravimetric analysis (TGA) characterizes the thermal stability of the copolymer. The initial decomposition temperature of C-PCPMI II was 270 oC. The gel permeation chromatography (GPC) determines the molecular weight and polydispersity index (PDI) as 8050 and 1.5191. The antimicrobial activities of monomer and the copolymer were also investigated against various microorganisms.