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Plants have wide range of medicinal values and have been used from ancient time to cure various kind of illnesses. India comes under the twelve mega-biodiversity countries of the world, which are rich in vegetation and have a wide variety of medicinal plants. From dawn of civilization, folks in India and China used medicinal plants to heal their wounds and various kinds of sicknesses. Codonopsis is a continual herb belongs to family Campanulaceae. Different studies depicted this plant as an important herb for various therapies. It is necessary to study this plant more broadly to know its medicinal values and use in the treatment of various diseases. Keeping this in view, in the present work green synthesis of silver nanoparticles using polyphenolic rich root extract of Codonopsis clematidea has been carried out. Characterization of synthesised nanoparticles has been carried out using UV-Visible, IR, XRD and TEM. The synthesized silver nanoparticles have also been evaluated for their biological potential.